| Date |
Tuesday, 29 October 2019, 17:00 onwards |
| Room |
7th Floor, Seminar Room 1, IDAC Center for Basic Aging Research |
| Title |
Perspective on HLA genome and gene analysis by NGS
|
| Speaker |
Takashi Shiina |
| Affiliation |
Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine
|
| Organizer |
Koetsu Ogasawara (Dept. Immunobiology・ext 8579) |
| Abstract |
The highly polymorphic Human Leukocyte Antigen (HLA) molecules (alleles) expressed by the different classical HLA class I and class II genes have crucial roles in the regulation of innate and adaptive immune responses. To date, over 23,000 HLA alleles have been published from IPD-IMGT/HLA database, and specific HLA alleles and HLA haplotypes have been reported to be associated with graft-versus-host disease (GVHD) of hematopoietic stem cell transplants, the pathogenesis of numerous infectious and/or autoimmune diseases and allergic reactions to various drugs. However, the HLA polymorphisms and HLA gene expression profiles have yet to be fully defined and understood in the processes of inflammatory and immune responses and autoimmune, chronic and infectious diseases. In recent years, various innovative techniques using next generation sequencer (NGS) have been developed for high-resolution level polymorphism and gene expression analyses of HLA genes, and they are expected to become a breakthrough for comprehensive understanding of the innate and acquired genetic factors of disease pathogenesis. In this seminar, I would like to introduce our developed NGS based HLA analysis methods and characteristics of DNA polymorphisms and RNA expression levels in Japanese population. |
