Secretariat, Alumni Association, IDAC
Date	Friday, 25 October 2019, 17:00 to 18:30
Room	7th Floor, Seminar Room 1, IDAC Center for Basic Aging Research
Title	High-resolution Analysis of Transcribed Enhancers Using a Novel 5’ Nascent RNA Sequencing
Speaker	Yasuhiro Murakawa
Affiliation	RIKEN Center for Integrative Medical Sciences
Organizer	Hozumi Motohashi (Dept. Gene Expression Regulation・ext 8550)
Abstract	Enhancers are activated in a highly cell-type specific manner, and play key roles in generating cell-type-specific transcriptomes. Capped transcripts are generated bi-directionally from active enhancers, but these transcripts are actively degraded by the nuclear exosome complex immediately after their synthesis. Here we developed a simple and robust NET-CAGE technology to profile 5’-ends of nascent RNAs in diverse cells and tissues, and we studied RNA synthesis and degradation at the transcription start site level. Sensitive and high-resolution analysis of enhancer-derived RNAs without the influence of RNA degradation allowed us to identify a number of new enhancers at an unprecedented depth, and to delineate primary locations of transcription within super-enhancers. I will talk about new insights into the dynamic and topological interplay between cis-regulatory elements.
Reference	Hirabayashi S., Bhagat S., Matsuki Y., Takegami Y., Uehata T., Kanemaru A., Itoh M., Shirakawa K., Takaori-Kondo A., Takeuchi O., Carninci P., Katayama S., Hayashizaki Y., Kere J., Kawaji H., Murakawa Y. NET-CAGE Characterizes Dynamics and Topology of Human Transcribed Cis-regulatory Elements. Nature Genetics, 51(9):1369-1379 (2019)