Secretariat, Alumni Association, IDAC
Date Friday, 28 July 2023, 16:00~
Room International Conference Room, Center for Smart-Aging Research, 1F (IDAC)
Title Transgenerationalinheritance of epigenetic signatures in mammals
Speaker Yuta Takahashi
Affiliation Altos Labs
Organizer Hiroki Sekine, Department of Gene Expression Regulation, ext 8553
Abstract Transgenerational epigenetic inheritance in mammals remains a debated subject. We recently demonstrated that DNA methylation of promoter-associated CpG islands (CGIs) can be transmitted from parents to their offspring in mice (Takahashi, Cell. 2023). We generated DNA methylation-edited mouse embryonic stem cells (ESCs) in which CGIs of two metabolism related genes were specifically methylated and silenced using our DNA methylation editing strategy (Takahashi, Science. 2017). DNA methylation-edited mice generated by microinjection of the methylated ESCs exhibited abnormal metabolic phenotypes. Both the acquired methylation of the targeted CGI and the phenotypic traits were maintained and transmitted across multiple generations. The heritable CGI methylation was subjected to reprograming in parental PGCs and subsequently reestablished in the next generation at post-implantation stages. In this meeting, I would like to discuss the mechanism underlying transgenerational epigenetic inheritance in mammals.

Takahashi Y. et al. Transgenerational inheritance of acquired epigenetic signatures at CpG islands in mice. Cell. 2023; 186: 4, 715-731

Takahashi Y. et al. Integration of CpG-free DNA induces de novo methylation of CpG islands in pluripotent stem cells. Science. 2017; 356: 503-508.