Secretariat, Alumni Association, IDAC
Date Wednesday, July 26, 2017 (16:00 to 18:00)
Room 7th Floor, Seminar Room 1, IDAC Center for Basic Aging Research
Title Core Mediator structure at 3.4 Å extends transcription initiation complex model
Speaker Kayo Nozawa
Affiliation Max-Planck-Institute
Organizer Toshihiko Ogura Dept. Developmental Neurobiology
Contact: Mariko Yoshida ・ext 8564
Abstract Mediator is a multi-sub unit co-activator of eukaryotic transcription that directly connects activator which is bound to regulatory DNA elements with RNA polymerase II. The Mediator system is conserved across the eukaryotic kingdom and is required for the majority of protein coding gene expression. In yeast, Mediator comprises 25 sub units with a total mass of ~1.4 MDa and is organized into four modules called the head, middle, tail and kinase. The head and middle modules form the essential cMed, whereas the tail and kinase modules play regulatory roles. Here, we report on the crystal structure of the 15-subunit core Mediator (cMed) at 3.4 Å resolution. These results provide a framework for understanding Mediator function in the transcription pre-initiation complex (PIC).